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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: FASEB J. 2007 Oct 30;22(3):910–917. doi: 10.1096/fj.06-7729com

Figure 4.

Figure 4

Cigarette smoke exposure results in aberrant phosphorylation of the EGFR. A) Serum-starved HAE cells were not treated (NT) or exposed to 100 ng/ml EGF for 15 min, 1 U/ml GO for 45 min, or smoke from 1 cigarette (CS) for 45 min. B) Serum-starved HAE cells were not treated (NT) or pretreated with 25 μM PP1 or PP2 for 45 min followed by treatment with smoke from 1 cigarette for 30 min in the absence or presence of the inhibitors as indicated. Cell lysates were separated by SDS-PAGE, transferred to nitrocellulose membranes and immunoblotted with the indicated EGFR phospho-tyrosine-specific Abs, anti-EGFR (RK2) Ab, or Abs against phospho-Src and total Src. p-Tyr, phosphotyrosine; p-Src, phospho-Src.