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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: FASEB J. 2007 Oct 30;22(3):910–917. doi: 10.1096/fj.06-7729com

Figure 5.

Figure 5

EGFR exposed to cigarette smoke cannot bind c-Cbl and is not ubiquitinated. Serum-starved HAE cells were untreated (NT) or incubated with 100 ng/ml EGF for 15 min, 1 U/ml GO for 30 min, or smoke from 1 cigarette (CS) for 45 min. Cells were lysed and A, the EGFR was immunoprecipitated (IP) from cell lysates using anti-EGFR mAb 225. B) EGFR was immunoprecipitated with anti-EGFR mAb 225 and Grb2 was immunoprecipitated with anti-Grb2 Ab. Immunoprecipitated proteins were separated by SDS-PAGE, transferred to a nitrocellulose membrane, and immunoblotted (IB) with the indicated Abs. Ub, ubiquitin; p-EGFR, phospho-EGFR.