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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: Methods Enzymol. 2014;541:13–26. doi: 10.1016/B978-0-12-420119-4.00002-1
Component Final concentration Stock Amount
Na-HEPES, pH 7.5 100 mM 1 M 10 ml
NaOAc, pH 7.5 400 mM 3 M 13.3 ml
EDTA 2 mM 0.5 M 40 μl
EGTA 2 mM 0.5 M 40 μl
MgOAc 10 mM 1 M 1 ml
Glycerol 10% 50% 20 ml
Add water to 100 ml