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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: Methods Enzymol. 2014;541:13–26. doi: 10.1016/B978-0-12-420119-4.00002-1
Component Final concentration Stock Amount
2× Lysis buffer 1× 2× 15 ml
NP-40 0.25% 10% 750 ml
DTT 3 mM 1 M 100 μl
PMSF 1 mM 100 mM 300 μl
Add water to 30 ml