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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: Mol Cancer Ther. 2012 Feb 1;11(4):795–804. doi: 10.1158/1535-7163.MCT-11-0698

Figure 2. EGFR directly interacts with c-Src (activated c-Src) under CS exposure.

Figure 2

Serum-starved A549 cells were exposed (or not) to 100 ng/ml EGF for 15 min. or to smoke from 1 cigarette for 30 min. EGFR was IPed from total cell lysates, separated by SDS PAGE, and IBed for total EGFR and Y-416 activated c-Src (p-Src). Each treatment and experiment/IP-IB was repeated three times. Sequential IBs of the immuno-precipitates were done by stripping the Abs off the membranes in between each IB. The images shown are representative of three independent experiments.