Figure 3. CS-induced activation of EGFR does not induce “classical” receptor dimerization.

Serum-starved A549 cells were exposed (or not) to 100 ng/ml EGF or exposed to smoke from ½ cigarette for 15 min. The cross linking reagent EDAC (1 mM) was added and incubation continued for an additional 15 min. 50 µg of total proteins from the cell lysates were separated by SDS-PAGE and IBed for total and Tyr-phosphorylated EGFR (p-EGFR). EGFR dimers and monomers are indicated. Each treatment and experiment/IP-IB was repeated three times. The images shown are representative of three independent experiments.