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. Author manuscript; available in PMC: 2015 Mar 23.
Published in final edited form as: Mol Cancer Ther. 2012 Feb 1;11(4):795–804. doi: 10.1158/1535-7163.MCT-11-0698

Figure 5. TKI-sensitive L858R EGFR MT becomes resistant to TKIs under CS exposure.

Figure 5

NIH-3T3 cells stably over-expressing the L858R EGFR MT were serum-starved and then incubated (or not) with A. 1 µM AG1478 or B. 1 µM Erlotinib for 30 min. Then, the cells were treated for 30 min. with 100 ng/ml EGF or exposed to smoke from 1 cigarette for 30 min. EGFR was IPed from the total cell lysates, separated by SDS-PAGE and IBed for total receptor, total Tyr-phosphorylation level (p-EGFR) and specific Tyr-residue phosphorylation level (Y845, Y1068, and Y1173). Each treatment and experiment/IP-IB was repeated three times. The images shown are representative of three independent experiments.