NIH-3T3 cells stably over-expressing the L858R EGFR MT were serum-starved and then incubated (or not) with A. 1 µM AG1478 or B. 1 µM Erlotinib for 30 min. Then, the cells were treated for 30 min. with 100 ng/ml EGF or exposed to smoke from 1 cigarette for 30 min. EGFR was IPed from the total cell lysates, separated by SDS-PAGE and IBed for total receptor, total Tyr-phosphorylation level (p-EGFR) and specific Tyr-residue phosphorylation level (Y845, Y1068, and Y1173). Each treatment and experiment/IP-IB was repeated three times. The images shown are representative of three independent experiments.