Skip to main content
. Author manuscript; available in PMC: 2016 Feb 27.
Published in final edited form as: J Mol Biol. 2014 Dec 19;427(4):887–900. doi: 10.1016/j.jmb.2014.12.008

Figure 1. Parent and hybrid species of SecB.

Figure 1

The four parent species and each of the hybrid tetramers generated are shown in cartoon form. The hybrids are designated by letters which denote the two species of parents that were mixed. The hybrids were stabilized by introduction of cysteines and formation of disulfide bonds between the dimers. The first letter is upper case and indicates the parent species that carried the Q12C substitution whereas the lower case letter in the second position indicates the parent that carried the A129C substitution. For example, the parents of hybrid Ba are SecBQ12C E77K (B) and SecBA129C (A, otherwise wild-type). Thus, the only difference between Dc and Cd is the Cys residue present in the parent.