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. Author manuscript; available in PMC: 2016 Feb 27.
Published in final edited form as: J Mol Biol. 2014 Dec 19;427(4):887–900. doi: 10.1016/j.jmb.2014.12.008

Figure 10. Relative Strength of interaction between Hybrid SecB species and SecA.

Figure 10

(a) The affinities are unitless, expressed relative to that of wild-type SecB without crosslink. Relative affinity determined from fit to F = R/(A+R). See legend to Fig. 8c. The affinity of Db for SecA relative to that of wild-type was determined by competition of hybrid Bb with 14C-SecBE77K. Hybrid Db had an affinity relative to that of Bb of 3.6; the relative affinity of Bb compared to 14C SecB wild type is 2.7. Thus, (3.6)(2.7) = 9.7. (b) Relative ΔG was calculated from the equation ΔG = −RTln (Kd−1) using the values of relative affinity in Table 1 as the relative Kd. The dot in the cartoon is plotted at the value of % ΔG using the energy of Cc as 100%.