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. Author manuscript; available in PMC: 2016 Feb 27.
Published in final edited form as: J Mol Biol. 2014 Dec 19;427(4):887–900. doi: 10.1016/j.jmb.2014.12.008

Figure 3. The six hybrid species.

Figure 3

Each of the hybrids (see cartoons in Fig. 1 for nomenclature) was analyzed by sodium dodecyl (14%) polyacrylamide gel electrophoresis. The samples in the first 6 lanes (reduced) were boiled 5 min. in sample buffer containing 1% SDS and 20 mM DTT before loading. Those in the 6 lanes on the right were boiled in sample buffer containing 1% SDS without DTT, but with 1 mM NEM (N-ethylmaleimide), which reacts with any free sulfhydryls, to avoid oxidative crosslinking.