Fig 4. Glucose lowering by insulin or its combination with cmpd1 is IR dependent.

Mice expressing IR-specific shRNA were fed with doxycycline in drinking water from day 1–11. Blood glucose (A) was monitored daily for 32 days during and post doxycycline treatment. Plasma insulin in doxycycline treated mice was examined on day 6, 12 and 24 in comparison with vehicle treated mice (B). n = 8 in each group. (C) Hyperglycemic IR-shRNA mice 15 days post doxycycline treatment received acute treatment of Cmpd1 (30 mg/kg) and insulin (1 U/kg) individually or in combination. A SGLT2 inhibitor (SGLT2i) was used as a control. Blood glucose was monitored hourly after dosing for 4 hours. ***p < 0.001 vs. vehicle-treated animals. The data are means ± SEM with n = 8 in each group.