Fig 6. Rat anti-rCsHK serum affects C. sinensis adult survival in vitro.
(A) The median survival of C. sinensis adults in the blank control group, 1:40 pre-immune serum group, 1:80 pre-immune serum group, 1:160 pre-immune serum group, 1:40 anti-rCsHK serum group, 1:80 anti-rCsHK serum group, and 1:160 anti-rCsHK serum group was 15, 8, 8, 9, 2, 3, and 3 days, respectively. There was no significant difference in survival rates among pre-immune serum groups at any dilution (p > 0.05). Significant differences were observed in the survival rates among the other groups (p < 0.05). (B) The enzymatic activity of CsHK in homogenate of parasites collected from each group at 1, 3, 5, and 6 days of incubation. The enzymatic activity of CsHK in adult worms incubated in medium with different dilutions of anti-rCsHK serum declined significantly in a dose- and time-dependent manner. (C) As a control, there was no obvious change of the enzymatic activity of CsPLA2 in the worms.
