Skip to main content
. 2015 Mar 23;10(3):e0118417. doi: 10.1371/journal.pone.0118417

Fig 2. Liver-specific overexpression of mouse miR-155 in transgenic mice mediated by Cre/lox P system.

Fig 2

(A) Strategy for liver-specific expression of miR-155 transgene using Cre/lox P system. In the absence of Cre-mediated recombination, only mRFP will be transcribed, while miR-155 and Luc transgene expression is prevented by STOP sequence flanked by lox P sites. When Cre-mediated recombination occurs in mouse liver, the floxed mRFP + 3×PolyA is excised, and miR-155 and Luc transgene expression is activated in a liver-restricted pattern in Rm155LG/Alb-Cre double transgenic mice. Other details as in Fig. 1A. (B–C) Whole-body bioluminescence (B) and fluorescence (C) imaging for newborn offspring derived from mating heterozygous Rm155LG transgenic mice with homozygous Alb-Cre mice. (D) In vivo luciferase imaging in the liver of both adult Rm155LG/Alb-Cre mouse and the control littermate developing from these offspring shown in Fig. 2B-C. (E-F) Ex vivo imaging of mRFP (E) and luc (F) expression in organs from same mouse shown [marked by asterisk (*)] in Fig. 2D. (G) qRT-PCR for mouse miR-155 transgene expression in liver from double transgenic mouse (Rm155LG/Alb-CreTg) and littermate control. *, P < 0.05 compared with control.