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. 2015 Mar 23;10(3):e0121305. doi: 10.1371/journal.pone.0121305

Fig 3. C. perfringens type E (CpE218) strain inhibits the growth of type A strain (Cp88).

Fig 3

Both strains were co-cultured under anaerobic conditions. (A) Optical density at 600 nm shows no differences (P>0.05) in total clostridia cell density between monocultures (Cp88) and co-cultures with inhibitor type E strain (CP88 + CpE218) and non-inhibitor strain (Cp88 + Cp31). (B) Samples were analyzed for nalidixic acid resistant (nalR) CFU/ml every 2 h of culture. Compared to the expected number of nalidixic acid resistant Cp88 grown in monoculture, there was a significant reduction in the number of nalidixic acid resistant CFU counts since 4 h of culture onwards. No significant differences were observed when cultivated with a nalidixic acid sensitive non-inhibitor type A strain (Cp31). Results are the mean CFU counts obtained from three independent experiments; error bars indicate the standard deviation (SD).