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. 2015 Mar 23;10(3):e0121305. doi: 10.1371/journal.pone.0121305

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© 2015 Redondo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) C. perfringens type E (CpE218) strain reduces adherence of type A strain (Cp88) to Caco-2 cells. Caco-2 cells were incubated with DMEM medium containing bacteria. Cp88: Nalidixic acid resistant type A strain alone; Cp88+Cp31: Mix of nalidixic acid resistant type A strain with a non-inhibitor type A strain but sensitive to nalidixic acid; Cp88+CpE218: Mix of nalidixic resistant type A strain with a type E strain. As mentioned, both strains were added simultaneously. Adhesion of Cp88 alone was considered as a control for the adhesion assay. (B) Type E cells displace type A cells attached to Caco-2 cells only at higher type A/type E ratio. The number below each column represents the ratio between attached type A and free competing type E cells. Results are the mean nalidixic acid resistant (nalR) CFU counts from three independent experiments; error bars indicate the standard deviation (SD).