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. 2015 Mar 23;10(3):e0121305. doi: 10.1371/journal.pone.0121305

Fig 5. Iota toxin increases type E adherence while decreases type A adherence.

Fig 5

(A) ITX treatment increases the adherence of C. perfringens type E (CpE218). Cells were incubated with the indicated toxin concentrations. 75ng/ml Ia +150ng/ml Ib, 150ng/ml Ia +300ng/ml Ib and 300ng/ml Ia +600ng/ml Ib. After ITX treatment, cells monolayers were incubated with type E bacteria. Cells were lysed and total clostridia were plated onto neomycin blood agar plates for counting. (B) ITX treatment increases the adherence of type E bacteria (CpE218), and reduce adherence of type A bacteria (Cp88—Cp31). Cells were treated with 150 ng/ml Ia and 300 ng/ml Ib (ITX), and washed 3 times with PBS. After ITX treatment, cells monolayers were incubated with the indicated strains. (C) ITX induced changes in bacterial adherence were neutralized by addition of anti-ITX IgY. Cells were treated with 150 ng/ml Ia and 300 ng/ml Ib (ITX) with or without neutralizing IgY. After treatment, cells monolayers were incubated with the indicated strains. Attachment levels were quantified as percent of bacterial adherence relative to untreated cells. Attachment levels were quantified as percent of bacterial adherence relative to untreated cells. Results shown represent the average of three independent experiments; error bars indicate the standard error of the mean (SEM).