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. 2015 Mar 23;10(3):e0118529. doi: 10.1371/journal.pone.0118529

Fig 1. PCR amplification of a portion of the 5’-UTR from GenMark eSensor RVP samples.

Fig 1

cDNA synthesized from RNA extracted from de-identified and masked RVP samples was used for traditional PCR amplification of the 5’-UTR. Primers were described by Oberste, et. al., for the sequencing of EV-D68 [2]. Agarose-ethidium bromide electrophoresis demonstrated that 49 of 62 samples analyzed yielded a 396 base pair amplicon compatible with EV-D68. Results shown corresponded to samples 2–4, 6–13, and 15–17, and are representative of the range of band intensities observed for all positive samples. NTC: no template control.