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. 2015 Mar 23;10(3):e0118678. doi: 10.1371/journal.pone.0118678

Fig 3. Estimation of the optimal number of reference genes required for accurate qPCR data normalization.

Fig 3

Estimation was done by calculating the pairwise variation (Vn/n+1) of the normalization factors NFn and NFn+1 as described in Vandesomple et al., [16].