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. 2015 Mar 23;10(3):e0119859. doi: 10.1371/journal.pone.0119859

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© 2015 Shabbir et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — a. Prostate cancer cells were treated with MEM for 24/48h, and cell viability was determined by MTT assay. Table shows the IC₅₀ of CWR22Rν1, C_4-2, PC-3 and DU145 at 24 and 48h. Mean ± SD of experiments performed in triplicate is shown. b. Dose-dependent effect of MEM on clonogenecity of CWR22Rν1 and C_4-2 cells as detected by colony formation assay. Details are described in material methods. c. Effect of various fractions (n-hexane, ethyl acetate, n-butanol and aqueous) on viability of CWR22R ν1 cells, determined by MTT assay. *p<0.05 and **p<0.01 was considered statistically significant.