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. 2015 Mar 23;10(3):e0121356. doi: 10.1371/journal.pone.0121356

Fig 5. Analyses of gangliosides by normal-phase LC-MS with regard to the glycan structure of gangliosides.

Fig 5

A, Scheme of synthesis pathway of a- and b-series of gangliosides. B, Representative chromatograms of normal-phase LC-MS of gangliosides using an NH2 column. GM1(d18:1–13C16:0) [m/z 1532.88 (shown on the left side on the chromatogram)] was used as ISD. In this system, a-series (GD1a) and b-series (GD1b) GD1-gangliosides were separately detected on the basis of the diversity of the glycan structure. The expression level of each ganglioside species was calculated by measuring each peak area above the baseline.