Skip to main content
. 2015 Mar 23;10(3):e0120318. doi: 10.1371/journal.pone.0120318

Table 1. Sedimentation equilibrium results for HIP2-Ub and Ubc1-Ub covalent complexes.

E2-Ub Complex Conc (μM) MWCalc (Da) MWObs a (Da)
Ubc1–UbCys 14.3 32791.1 35238 ± 265
21.5 32791.1 32536 ± 212
43.0 32791.1 31347 ± 222
HIP2–UbCys 7.2 31163.5 34461 ± 219
14.4 31163.5 34220 ± 169
24.0 31163.5 34619 ± 175

a Molecular weight determined using global fits of 15k, 18k, 22k and 26k rpm to a single species model.