Table 1. Sedimentation equilibrium results for HIP2-Ub and Ubc1-Ub covalent complexes.
E2-Ub Complex | Conc (μM) | MWCalc (Da) | MWObs a (Da) |
---|---|---|---|
Ubc1–UbCys | 14.3 | 32791.1 | 35238 ± 265 |
21.5 | 32791.1 | 32536 ± 212 | |
43.0 | 32791.1 | 31347 ± 222 | |
HIP2–UbCys | 7.2 | 31163.5 | 34461 ± 219 |
14.4 | 31163.5 | 34220 ± 169 | |
24.0 | 31163.5 | 34619 ± 175 |
a Molecular weight determined using global fits of 15k, 18k, 22k and 26k rpm to a single species model.