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(A) Protein expression of SIRT1 or SIRT1 (H363Y) in C2C12 myoblasts; (B) acetyl-p53 (K376) in C2C12 myoblasts overexpressing empty vector, SIRT1 or SIRT1 (H363Y). *p<0.01 vs empty vector; ‡ p<0.01 vs SIRT1 (H363Y), one-way ANOVA. En, endogenous SIRT1 protein; OE, overexpressed SIRT1 protein. Data are expressed as means ± SEM. n = 3 replicate experiments done in triplicate.
