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. 2015 Mar 23;10(3):e0119467. doi: 10.1371/journal.pone.0119467

Fig 2. Neurospheres can be generated from YFP+ve, YFP-ve and mixed gut cell populations.

Fig 2

Panels show phase contrast images of cultures containing neurospheres and their YFP fluorescent counterparts. (A-D) Neurospheres from unsorted gut cells contain both YFP+ve and YFP-ve cells whereas neurospheres derived from YFP+ve sorted cells (E-H) contain only YFP+ve cells. (I-L) Neurospheres from cultures of YFP-ve, non-neural crest cells do not contain YFP+ve cells. Neurospheres are similar in size and appearance in all cases. Scale bar in G = 200μm (applies to A-C, E-G, I-K) and in H = 40μm (applies to D, H, L).