Fig 7. Neurospheres derived from postnatal human gut cultures are heterogeneous in composition.
(A) Bright field image of neurosphere derived from human postnatal gut cultured for 2 weeks. Neurospheres contain neural crest-derived cells as shown by expression of the neural crest cell marker p75 (B) and the neuronal marker TuJ1 (C). Neurospheres also contained non-neural crest cell derivatives such as fibroblast-like cells expressing PDGFRα (D), and muscle cells expressing SMA (E). Proliferating cells are labelled with Ki67 (F). Following FACS sorting based on selection with p75NTR, neurospheres were generated exclusively from p75-ve (G) and p75+ve cells (H) similar in shape and appearance. DAPI (blue) stains nuclei in B-F. Scale bar = 50μm.