Skip to main content
. 2015 Mar 23;10(3):e0119912. doi: 10.1371/journal.pone.0119912

Fig 2. TRPM7 is down-regulated in mouse cortical astrocytes by siRNA.

Fig 2

A. RT-PCR shows that transfection of astrocytes with specific TRPM7 siRNA decreases the mRNA level of TRPM7 compared to those with control siRNA. However, the mRNA levels of homologous genes TRPM4 and TRPM6 were not changed. B. Quantification of the expression of TRPM7, TRPM4 and TRPM6 mRNA in astrocytes treated with control siRNA or TRPM7 siRNA. Normalized TRPM7 mRNA expression shows a significant decrease after transfection with TRPM7 siRNA-1 (by 71.6% ± 11.9%) and siRNA-2 (by 70.2% ± 8.9%) as compared to control siRNA (*, p<0.05, n = 3). TRPM4 and TRPM6 mRNA levels were not changed (n = 3). C. Western blotting shows that TRPM7 siRNA-1 and siRNA-2 decrease the protein level of TRPM7 in astrocytes as compared to control siRNA. D. Quantification of TRPM7 protein level in astrocytes transfected with control siRNA and TRPM7 siRNAs. TRPM7 siRNA-1 significantly decreases TRPM7 protein expression by 50.9% ± 11.7%, while TRPM7 siRNA-2 decreases TRPM7 protein by 36.4% ± 6.1% (*, p < 0.05, n = 3). E. Quantitative RT-PCR confirms the reduction of TRPM7 mRNA normalized to β-actin in astrocytes transfected with TRPM7 siRNA-1 compared to those with control siRNA (*, p < 0.05, n = 3).