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. 2015 Mar 23;10(3):e0119508. doi: 10.1371/journal.pone.0119508

Fig 2. Evidence for extracellular glycosylation of CelA.

Fig 2

Panel A, Coomassie stained gel. Panel B, the same gel stained with Glycoprotein Stain (G-Biosciences). Lane1, cell free extract from JWCB040 ΔpyrFA pDCW170::celA (no signal sequence); Lane 2, cell free extract from JWCB046 ΔpyrFA ldh::ISCbe4 Δcbe1 ΔcelA pDCW173::celA (with the signal sequence); M, Molecular weight markers (Biorad); Lane 3, supernatant from JWCB029 ΔpyrFA ldh::ISCbe4 Δcbe1 ΔcelA; Lane 4, JWCB046 ΔpyrFA ldh::ISCbe4 Δcbe1 ΔcelA pDCW173::celA (with the signal sequence).