Fig 2. Apoptosis in kidney tissue 24 hr after LPS challenge.

(A) A representative terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining in the mouse kidneys using the QIA33 FragEL DNA Fragmentation Detection Kit (EMD Millipore, Billerica, MA). TUNEL-positive nuclei (dark brown) in representative kidney sections are indicated by arrows. Magnification: 40x (scale bar, 100 μm). (B) The panel represents the percentage of TUNEL-positive nuclei from different groups. The TUNEL-positive nuclei in nine fields (500 μm²/field) from three separate sections of each kidney sample were counted and plotted as the average number of nuclei per mm². Data are expressed as mean ± SEM (N = 3 mice per group), *P<0.05.