Table 1. List of murine gene specific primers and probes* for RT-PCR.
| Gene | Primers | Sequence (5’ to 3’) |
|---|---|---|
| β-actin | Forward primer | AGAGGGAAATCGTGCGTGAC |
| Reverse primer | CAATAGTGATGACCTGGCCGT | |
| Probe | CACTGCCGCATCCTCTTCCTCCC | |
| IL-6 | Forward primer | CTTCAACCAAGAGGTAAAAGATTTA |
| Reverse primer | TAGCCACTCCTTCTGTGACTCC | |
| Probe | CCTTCCTACCCCAATTTCCAATGCTCTCCTA | |
| TNF-α | Forward primer | CCCCAAAGGGATGAGAAGTTC |
| Reverse primer | GCTTGTCACTCGAATTTTGAGAA | |
| Probe | TCATCAGTTCTATGGCCCAGACCCTCA | |
| MCP-1 | Forward primer | GTTGGCTCAGCCAGATGCA |
| Reverse primer | AGCCTACTCATTGGGATCATCTTG | |
| Probe | TTAACGCCCCACTCACCTGCTGCTACT | |
| KC | Forward primer | CTGGGATTCACCTCAAGAACATC |
| Reverse primer | CAGGGTCAAGGCAAGCCTC | |
| Probe | TTGCCCTCAGGGCCCCACTG | |
| ICAM-1 | Forward primer | GGAGGTGGCGGGAAAGTT |
| Reverse primer | AGGTCCAGTTCCCCAAGCA | |
| Probe | CGTGCTGTATGGTCCTCGGCTGGA | |
| PAI-1 | Forward primer | GACACCCTCAGCATGTTCATC |
| Reverse primer | AGGGTTGCACTAAACATGTCAG | |
| Probe | TCCTGCCTAAGTTCTCTCTGGAGACTGAAG | |
| IL-10 | Forward primer | GGTGTCCTTTCAATTGCTCTCAT |
| Reverse primer | ATCAAAGGATCTCCCTGGTTTCT | |
| Probe | ACCCATGAGTTTCTTCACAACTCTCTTAGGAGCT |
*The probe was labeled at the 5’-end with the reporter dye, 6FAM, and at the 3’-end with the quencher dye, BHQ1a (Eurofins Scientific, Huntsville, AL).