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. 2015 Mar 23;10(3):e0120296. doi: 10.1371/journal.pone.0120296

Fig 5. ZEB2 intronic sense transcripts manifest disparate expression patterns during development in mice.

Fig 5

A) Intronic riboprobe to regions 2a, 2g, and 2h within ZEB2 gene were used to detect sense-strand RNAs in mouse embryo head at E14 (B-E). Two different exon regions were used as internal controls, one within 3’ UTR (B) and another corresponding to the cDNA riboprobe used in the Allen Mouse Brain Atlas at E16 (F). B-I) RNA in situ hybridization in sagittal head sections of E14 and E16 mouse embryos. Scale bar E14 = 0.5 mm; E16 = 1 mm. J-K) Sagittal images of the olfactory bulb of E16 embryos highlighting the complementary expression pattern of ZEB2 in the olfactory nerve (ON), and Zeb2:2h in the mitral (MCL) and granule (GCL) cell layers. Scale bar = 100 μm. L. Sagittal section, RNA in situ hybridization detecting region Zeb2:2h in the adult mouse brain. Scale bar = 1 mm. BS, brain stem; CE, cerebellum; CP, caudoputamen; CxP, cortical plate; GE, ganglionic eminence; HIP, hippocampus; HY, hypothalamus; MB, midbrain; OB, olfactory bulb, OE, olfactory epithelium; OT, otic sensory epithelium; PC, progenitor cells of the cerebral cortex; PIT, pituitary gland; TG, trigeminal gland, TH, thalamus.