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. 2015 Mar 23;10(3):e0119235. doi: 10.1371/journal.pone.0119235

Fig 5. C. muridarum-induced IFN-β affects the gene expression levels of components found in the type-1 IFN signaling pathway.

Fig 5

Bm1.1 cells were infected with 10 IFU/ cell C. muridarum, and the gene expression levels of IFN-β, TLR3, IRF3, IRF7, and Stat1 were measured by RT-qPCR after total cell mRNA was harvested at either early (12h) or late (24h) times post infection. (A) Transcription results of Bm1.11 OE cells harvested at 12h PI to measure the impact on transcription of candidate genes after cells were incubated from 4-to-12h PI in culture medium containing 1μg/ml of either IFN-β neutralizing antibody (α-IFNβ) or isotype control antibody (α-IgG). (B) Transcription results of these genes after cells were incubated in culture medium containing either antibody from 12-to-24h PI. The results shown are representative of three independent experiments; Fold change is compared to Mock-infected controls.