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. 2015 Mar 23;10(3):e0119235. doi: 10.1371/journal.pone.0119235

Table 1. Primers for RT-qPCR.

Sense Primer Antisense Primer
IFN-β 5ʹ˗AAGAGTTACACTGCCTTTGCCATC˗3ʹ 5ʹ˗CACTGTCTGCTGGTGGAGTTCATC˗3ʹ
IFNα-2 5ʹ˗AGCAGATCCAGAAGGCTCAA˗3ʹ 5ʹ˗CATTCCAAGCAGCAGATGAA˗3ʹ
IFNα-4 5ʹ˗TTCTGCAATGACCTCCATCA˗3ʹ 5ʹ˗TATGTCCTCACAGCCAGCAG˗3ʹ
STAT1 5ʹ˗CGGAGTCGGAGGCCCTAAT˗3ʹ 5ʹ˗ACAGCAGGTGCTTCTTAATGAG˗3ʹ
STAT2 5ʹ˗TTTGGCTACCTGGATTGAAGAC˗3ʹ 5ʹ˗GGCTGAATTTTCGCAAGTTATGC˗3ʹ
IRF3 5’-TGGACGAGAGCCGAACGAGGTT-3’ 5’-CGAACTCCCATTGTTCCTCAGC-3’
IRF7 5ʹ˗CAATTCAGGGGATCCAGTTG˗3ʹ 5ʹ˗AGCATTGCTGAGGCTCACTT˗3ʹ
β-actin 5ʹ˗GGCTGTATTCCCCTCCATCG˗3ʹ 5ʹ˗CCAGTTGGTAACAATGCCATGT˗3ʹ
IFNAR1 5’-ACACAGTTTCGTGTCAGAGCA-3’ 5’- AACGGATCAACCTCATTCCAC-3’
TLR1 5’-GTGAATGCAGTTGGTGAAGAAC-3’ 5’-GCTCATTGTGGGACAAATCCAA-3’
TLR2 5’-CTTGTTTCTGAGTGTAGGGGCT-3’ 5’-CGAACCAGGAGGAAGATAAACT-3’
TLR3 5′-CCCGTTCCCAACTTTGTAGATG-3′ 5′-TGCCAAATACTCCCTTTGTTGAA-3′
TLR5 5’-CAGTATCAGCTGATGAGACATGAG-3’ 5’-GACAGTACCGCAATAGGGATGG-3’
TLR6 5’-TACGGAGCCTTGATTTCCATGT-3’ 5’-TGGACCTCTGGTGAGTTCTGAT-3’
IL-6 5’-CTATACCACTTCACAAGTCGGAGG-3’ 5’-TGCACAACTCTTTTCTCATTTCC3–3’
GM-CSF 5’-GCCATCAAAGAAGCCCTGAA-3’ 5’-GCGGGTCTGCACACATGTTA-3’
CCL4 5’-CCCACTTCCTGCTGTTTCTC-3’ 5’-GAGGAGGCCTCTCCTGAAGT-3’
CCL5 5’-ACTCCCTGCTGCTTTGCCTAC-3’ 5’-GAGGTTCCTTCGAGTGACA-3’
CXCL9 5’-GAGTTCGAGGAACCCTAGTGATAAGGAA-3’ 5’-AGGTTTGATCTCCGTTCTTCAGTGTAGC-3’
CXCL10 5’-CGATGACGGGCCAGTGAGAATG-3’ 5’-TCAACACGTGGGCAGGATAGGCT-3’
IL-1α 5’-AGGAGAGCCGGGTGACAGTA-3’ 5’-AACTCAGCCGTCTCTTCTTCAGA-3’
TNFα 5’-TGTGGCTTCGACCTCTACCTC-3’ 5’-GCCGAGAAAGGCTGCTTG-3’
Omp1 5’-GCCGCTTTGAGTTCTGCTTCCTC-3’ 5’-CCAAGTGGTGCAAGGATCGCA-3’
16S rRNA 5’-GCAAGTCGAACGGAGCAATT-3’ 5’-ACCCTTCCGCCACTAAACA-3’