Table 1. Comparison of the sequence types of B. afzelii strains according to multi locus sequence typing (MLST), and ospA and ospC typing.

Organism	MLST	ospA	ospC
Baf_K78	ST335	3 (2)	A5
Baf_ACA-1	-	3 (2)	A1
Baf_PKo	ST71	1 (2)	A2
Baf_HLJ01	ST106	-	-
Bbu_B31	ST1	9 (1)	B4

MLST typing, according to the system described by Margos et al.[54] comprising 592 defined profiles, assigned K78 to sequence type ST335 which is identical to the Italian strains 0600839I and 05001891I in the Borrelia MLST database [55]. No match for ACA-1 and Tom3107 was found in the MLST data base with their respective sequence profiles. Column ospA lists the ospA sequence type from the MLSA database [56] and in parentheses the OspA serotypes. The nearest hit for Tom3107 is ospA sequence type-3 with 1 bp mismatch: ospC classification follows the scheme of Seinost et al. [57] and Lagal et al. [58]. Tom3107 do not fall into any invasive group. For strain HLJ01 only the chromosome sequence is available.