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. 2015 Mar 23;10(3):e0119823. doi: 10.1371/journal.pone.0119823

Fig 2. Engineered differences in 5’ mRNA ΔG do not alter ply mRNA quantity.

Fig 2

Fold difference in ply mRNA levels (y axis) was calculated relative to wild-type (ΔG = -7.3) at early exponential phase. Relative mRNA amounts were calculated by ΔΔCT normalized to pneumococcal 16S rRNA. (N.S. = not significant; ANOVA)