Fig 2. Engineered differences in 5’ mRNA ΔG do not alter ply mRNA quantity.
Fold difference in ply mRNA levels (y axis) was calculated relative to wild-type (ΔG = -7.3) at early exponential phase. Relative mRNA amounts were calculated by ΔΔCT normalized to pneumococcal 16S rRNA. (N.S. = not significant; ANOVA)