Table 3.
Effect of various alternative electron acceptors on the activity of GalOx.
| Electron acceptor | Conc. stock (mM) | Amax (nm) | Absorption coefficient (mM−1 cm−1) | Relative activity (%) |
|---|---|---|---|---|
| O2 | Air | 420 | 43.2 | 100 |
| ABTS cation radical | 1 | 420 | 43.2 | 0 |
| Ferrocenium ion | 1 | 300 | 4.3 | 0 |
| 1,4-Benzoquinone | 10 | 290 | 2.24 | 0 |
| 2,6-Dichloro-indophenol (DCIP) | 3 | 600 | 11.8 | 0 |
| Ferricyanide | 4 | 420 | 0.98 | 0 |
| Guaiacol radical | 10 | 465 | 12.1 | 0 |
| 2,6-Dimethoxyphenol radical | 10 | 469 | 49.6 | 0 |
| Caffeic acid radical | 10 | 315 | n.d. | 0 |
| p-Coumaric acid radical | 10 | 285 | n.d. | 0 |
| Ferulic acid radical | 10 | 285 | n.d. | 0 |
| Sinapic acid radical | 10 | 305 | n.d. | 0 |
| Thioflavin T | 10 | 220, 400, 490, 950 | n.d. | 0 |
| 2-(4′-Methylaminophenyl)benzothiazole (BTA-1) | 10 | 350 | n.d. | 0 |
| 1,1′-Diethyl-2,2′-carbocyanine iodide | 10 | 420, 614 | n.d. | 0 |