Table 1. Comparison between the TBI and the Poisson-Boltzmann predictions and test against experimental data.

RNA or DNA	Comparison of parameters	Reference	The average error for BP	The average error for TBI
Three DNA helices	Folding free energy	Fig. 2d [22]	1.2 kcal/mol	0.1 kcal/mol
Two DNA helices	Melting temperature	Fig. 5 [23]	5.4°C	1.0°C
Two RNA helices	Ion binding Fraction	Fig. 2a [23]	0.11	0.06
24bp B-DNA helix	Ion binding Fraction	Fig. 2a [24]	0.08	0.01
40bp A-RNA helix	Ion binding Fraction	Fig. 2c [24]	0.15	0.04
40bp B-DNA helix	Ion binding Fraction	Fig. 2e [24]	0.12	0.03
BWYV pseudoknot RNA	Ion binding Fraction	Fig. 3a [24]	0.05	0.03
58-nt rRNA	Ion binding Fraction	Fig. 3c [24]	0.10	0.03
Yeast tRNAPhe	Ion binding Fraction	Fig. 3e [24]	0.06	0.03