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. 2015 Mar 23;9(3):e0003627. doi: 10.1371/journal.pntd.0003627

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© 2015 Nausch et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Human PBMC were gated on live cells using forward (FSC) and side (SSC) scatter. Gated live cells were analysed using the area against height of the FSC to discriminate singlet’s from duplicates. Singlet’s were subsequently analysed by a lineage cocktail (Lin) and CD45 to gate on Lin-CD45+ cells (as indicated in the box) (B). Lin-CD45+ cells were gated on CD127+ cells, which were analysed for CD294 and CD161 (C). CD127+CD294+CD161+ were analysed for CD117 (grey histogram) versus isotype control (open histogram) (D). Flow charts as presented are from the PBMC analysis of a 9 year old female who was negative for S. haematobium eggs.