Fig 12. Sensitivity of dsRNA detection in comparison to viral protein of circulating arboviruses at different time points.
A) C6/36 cells were infected with WNVKUNV or B) RRV T48 in serial dilutions from 10-1 to 10-12 and fixed with acetone at 24, or 48 hours post-infection or at 5 days post-infection. Fixed cell ELISA was performed using antibodies 3G1, 2G4 and the virus E protein-specific mAbs 4G2 (flavivirus) or G8 (RRV, alphavirus). Sensitivity is presented as TCID50 values for virus detected by each antibody. *** denotes significant difference between level of virus detection by mAbs 3G1 or 2G4 and virus-specific mAb 4G2 or G8. Statistical significance was analysed using one-way ANOVA comparison of means, followed by Turkey test for multiple comparisons (P<0.0005).