Table 2. Detection of circulating arboviruses from mosquito homogenates by 3G1 and 2G4.

Pool ID	Year	Original processing results		New processing results
		Virus Identified*	CPE	3G1/2G4 ELISA #	No. of inoculated wells positive (out of 4)	Detection and sequence confirmation by RT-PCR &
174	1996	SINV	+	+	4	+
487	1997	SINV	+	+	1	+
494	1997	SINV	+	+	4	+
495	1997	SINV	+	+	4	+
496	1997	SINV	+	+	4	+
979	1998	Negative	- 1	+	4	-
981	1998	Negative	- 1	+	4	-
982	1998	Negative	- 1	+	4	-
1014	1998	KOKV	+	+	4	+
1026	1998	KOKV	+	-	0	-
1049	1998	KOKV	+	+	4	-
1051	1998	KOKV	+	+	4	-
1053	1998	KOKV	+	+	4	+
1197	1999	WNVKUNV	+	+	4	+
1268	1999	WNVKUNV	+	+	3	+
1274	1999	WNVKUNV	+	+	4	+
1284	1999	ALFV	+	+	4	+
1391	1999	WNVKUNV	+	+	4	+
1490	1999	WNVKUNV	+	+	3	+
1628	1999	WNVKUNV	+	+	4	+
1686	1999	ALFV	+	+	4	+
2116	2000	WNVKUNV	+	+	4	+
2129	2000	WNVKUNV	+	+	4	+
2206	2000	WNVKUNV	+	+	4	+
2269	2000	ALFV	+	+	4	+
2279	2000	WNVKUNV	+	+	4	+
2472	2000	WNVKUNV	+	+	4	+
3342	2000	WNVKUNV	+	+	1	+
3404	2000	WNVKUNV	+	+	4	+
3618	2000	WNVKUNV	+	+	3	+
3740	2000	WNVKUNV	+	-	0	+
3777	2000	SINV	+	+	1	-
4009	2000	SINV	+	+	4	-
4079	1999	WNVKUNV	+	+	1	+
4189	1999	ALFV	+	-	0	+
4270	2001	Negative	- 1	+	4	-
4271	2001	Negative	-	+	4	-
4280	2001	Negative	-	-	-	-
4298	2001	Negative	-	+	4	-
4309	2001	Negative	-	-	0	-

Legend: + positive;—negative.

* Viruses previously identified using virus specific monoclonal antibodies in ELISA

# Fixed-cell ELISA using mAbs 3G1 and 2G4 against four wells of C6/36 cells inoculated with mosquito homogenate. Samples were deemed positive if at least one well gave strong positive in ELISA.

^&RT-PCR using flavivirus- and alphavirus-specific primers was performed on RNA extracted from supernatant harvested from inoculated wells prior to fixing.

¹CPE was observed in these samples upon subsequent passaging during our analysis.

Negative, no virus was identified by reagents specific for known viruses; SINV, Sindbis virus; WNV_KUNV, West Nile virus subtype Kunjin; ALFV, Alfuy virus; KOKV, Kokobera virus.