Table 3. Novel virus isolates from mosquito homogenate detected in ELISA by mAbs 3G1 and 2G4.
Pool no. | Sequence length (bp) | Positive ELISA optical density reading * | RT-PCR primer or Next generation sequencing (NGS) | E-value | Identity | Query cover | Closest match | Genbank accession no. |
---|---|---|---|---|---|---|---|---|
1 | 863 | 1.057 | NGS | 8e-106 | 68% | 95% | Corriparta virus (Reoviridae) | AGT51062.1 |
2 | 248 | 1.298 | RT-PCR | 1e-11 | 61% | 53% | Uncultured virus (RdRP-like protein) | AEA40853.1 |
3 | 2785 | 0.863 | NGS | 0 | 98% | 94% | Liao Ning Virus (Reoviridae) | AAQ83563.1 |
4 | 601 | 0.6 | RT-PCR | 2e-113 | 86% | 98% | Mediterranean Ochlerotatus flavivirus (Flaviviridae) | AEH43703.1 |
Legend: Preliminary sequence analysis performed using BLASTX
* OD of test sample with mock OD subtracted