Table 3. Novel virus isolates from mosquito homogenate detected in ELISA by mAbs 3G1 and 2G4.

Pool no.	Sequence length (bp)	Positive ELISA optical density reading *	RT-PCR primer or Next generation sequencing (NGS)	E-value	Identity	Query cover	Closest match	Genbank accession no.
1	863	1.057	NGS	8e-106	68%	95%	Corriparta virus (Reoviridae)	AGT51062.1
2	248	1.298	RT-PCR	1e-11	61%	53%	Uncultured virus (RdRP-like protein)	AEA40853.1
3	2785	0.863	NGS	0	98%	94%	Liao Ning Virus (Reoviridae)	AAQ83563.1
4	601	0.6	RT-PCR	2e-113	86%	98%	Mediterranean Ochlerotatus flavivirus (Flaviviridae)	AEH43703.1

Legend: Preliminary sequence analysis performed using BLASTX

* OD of test sample with mock OD subtracted