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. 2015 Mar 23;10(3):e0122555. doi: 10.1371/journal.pone.0122555

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© 2015 Sie et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — Cell viability assays demonstrating additional effects of combination treatment in pediatric low grade astrocytoma (Res-259) and ependymoma (Res-196) compared with single treatment (mean ± SD, 48h). Immunoblots showing effect of single RTK inhibition (8 uM), the rescue effect of growth factors (100 ng/ml) and the ability of the second inhibitor (canertinib 4 uM, crizotinib 3 uM, sorafenib 2 uM) to overcome this growth-factor-driven rescue on Akt and Erk phosphorylation (p) in pediatric low grade astrocytoma and ependymoma cells (2h). Protein expression was normalized to β-actin as loading control and derived from three independent experiments of which the mean (± SEM) was calculated. The ability of crizotinib to overcome HGF-driven rescue during canertinib treatment reached statistical significance on Akt and Erk phosphorylation (p = 0.028 and p = 0.046 respectively).