A, qRT-PCR analysis of TRIM24 mRNA and Immunoblot of TRIM24 and p53 in Control, TRIM24-1, and TRIM24-2 iHMECs. B, Cell proliferation analysis of Control, TRIM24-1, and TRIM24-2 iHMECs. C, Cell-cycle analysis of Control, TRIM24-1, and TRIM24-2 iHMECs. D, Anchorage-independent proliferation of Control iHMECs, TRIM24-1 iHMECs, and MCF-7 cells as determined using a soft agar colony formation assay (left) and quantification of the colonies per field ± SD (right). The data are averages from two replicates ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.