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. 2015 Mar 23;10(3):e0121657. doi: 10.1371/journal.pone.0121657

Table 1. Details of immunohistochemical staining used in these studies.

Cell Type Primary Ab Fixative
Gonadotrophs Guinea Pig anti-FSHβ (NIDDK-NIH) Bouins
Gonadotrophs Mouse anti-LHβ (Gift from Dr. W. Colin Duncan) 10% NBF
Thyrotrophs Guinea Pig anti-TSHβ (AFP3035990P, NIDDK-NIH), gift from Prof. Alan McNeilly Bouins
Corticotrophs Mouse anti-ACTH (#M3501, DAKO) Bouins
Somatotrophs Rabbit anti-GH (A0570, DAKO) Bouins
Lactotrophs Rabbit anti- PRL (A0569, DAKO) Bouins
AR+ve Cells Rabbit anti-AR (ab74272, Abcam) Bouins or 10% NBF
ERα+ve cells Rabbit anti-ERα (MC-20) (Santa Cruz #sc-542) Bouins
YFP/GFP Rabbit anti-YFP/GFP (Life Technologies A-11122) Bouins