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. 2015 Mar 18;10:2155–2171. doi: 10.2147/IJN.S72923

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© 2015 Lambadi et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Live/Dead staining of planktonic Pseudomonas aeruginosa PAO1 cells by flow cytometer and FM.

Notes: Flow cytometer scatter plot for (A) untreated and unstained P. aeruginosa cells, (B) untreated SYTO9/PI stained, and (C) PBSNPs MIC-treated P. aeruginosa PAO1 cells stained with SYTO9/PI stains was analyzed; LL shows the unstained cells, UL region represents live cells, and dead cells are seen in the UR region. FM of cells prepared (D) untreated stained cells and (E) PBSNPs MIC-treated stained cells (i) represents live cells stained as green, (ii) dead cells stained as red.

Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin; FM, fluorescence microscopy; PBSNPs, polymyxin B-capped silver nanoparticles; MIC, minimum inhibitory concentration; LL, lower left; LR, lower right; PI, propidium iodide; UL, upper left; UR, upper right.