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. 2015 Mar 24;5:9248. doi: 10.1038/srep09248

Figure 1. Exogenous but not endogenous ephrin-B ligand can activate EphB2 in EphB2 overexpressing Ink4a/Arf(−/−) STeNSCs (utNSCs).

Figure 1

(a) Ephrin-B3 immunoblot of uninfected Ink4a/Arf(−/−) STeNSCs (lane 3) or infected with control (pCX4-RedEX; lane 1) or EphB2 expressing (lane 2) retrovirus. (b) In vitro EphB2 activation assay of utNSCs incubated for 24 hrs. with anti-human-IgG (lane1), ephrin-B1 (lane 2), ephrin-B2 (lane 3), or ephrin-B3 (lane 4). (c) Immunoblot of phospho- and total C-Raf, SAPK/JNK and p44/42 of utNSCs incubated with anti-human-IgG (lane1), and ephrin-B2 or ephrin-B3 for 15, 30, 60, and 120 minutes.