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. 2015 Mar 24;5:9430. doi: 10.1038/srep09430

Figure 2. miRNA recovery of different extraction kits were evaluated by the Cq values for spike-in controls (cel-miR-39 and cel-miR-54) assayed in the (a) TaqMan and (b) miScript RT-qPCR system.

Figure 2

Box plots with whiskers show 5–95 percentile of the Cq values for spike-in controls and the corresponding CVs are shown. The extent of extraction bias across samples was reflected in the range and CV of Cq values for spike-in controls. Higher median Cq compared to other extraction kits indicate poorer performance in miRNA recovery. Q, miRNeasy Serum/Plasma kit; E, miRCURY™ RNA Isolation Kit - Biofluids; A, mirVana™ PARIS™ Kit; MN, NucleoSpin® miRNA Plasma; NB, Plasma/Serum Circulating RNA Purification Kit.