Figure 5. Comparison of TaqMan and miScript RT-qPCR systems adapted to the high-throughput platform by performance parameters which include reproducibility, accuracy and sensitivity.

Transformation of data to z-score was carried out to facilitate direct comparison. A1, accuracy when measuring fold change of abundant miRNAs; A2, accuracy when measuring fold change of less abundant miRNAs, R1, reproducibility of RT replicates; R2, reproducibility of RT replicates after normalisation; R3, reproducibility of qPCR replicates; S1, sensitivity to detect the presence of plasma miRNAs and S2, sensitivity to detect the presence of miRNAs in titration points.