Table 2.

Summary of results from MCSA assignment simulations on MLKL N-terminal domain (PDB 2MSV)

chemical shift uncertainties (ppm)	number of 3D spectra	ambiguous residue-type assignments?	“invisible” segments?	number of successful runs from 10 attempts	maximum number of incorrect NCACX assignments in successful runs
0.1	4	no	no	10	0
0.2	4	no	no	10	4
0.3	4	no	no	5	6
0.4	4	no	no	2	8
0.6	4	no	no	0	-
0.8	4	no	no	0	-
0.1	3	no	no	10	0
0.2	3	no	no	10	4
0.3	3	no	no	10	12
0.4	3	no	no	10	14
0.6	3	no	no	5	26
0.8	3	no	no	2	48
0.1	2	no	no	10	4
0.2	2	no	no	10	8
0.3	2	no	no	9	22
0.4	2	no	no	10	26
0.6	2	no	no	0	-
0.8	2	no	no	1	87
0.1	4	yes	no	10	0
0.2	4	yes	no	10	4
0.3	4	yes	no	7	12
0.4	4	yes	no	1	8
0.6	4	yes	no	0	-
0.8	4	yes	no	0	-
0.1	4	no	yes	10	0
0.2	4	no	yes	10	4
0.3	4	no	yes	5	12
0.4	4	no	yes	3	8
0.6	4	no	yes	0	-
0.8	4	no	yes	0	-