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. 2015 Apr;22(4):197–202. doi: 10.1101/lm.036590.114

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© 2015 Vecsey et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Sleep deprivation causes similar changes in hippocampal gene expression in young and aged mice. Young (2 mo) and aged (22–23 mo) C57BL/6 NIA mice were sleep-deprived for 5 h by gentle handling. Immediately afterward, hippocampal tissue was dissected and analyzed by quantitative real-time RT-PCR for the expression of several genes that had previously been shown to be up- or down-regulated by SD in the hippocampus of young mice (Vecsey et al. 2012). (A) Up-regulated genes. There was an overall effect of SD across both age groups on each gene. No significant effects of age were observed. The exception was Hspb1, for which there was a significant interaction between age and SD, indicating that SD caused a smaller up-regulation in aged animals than in young animals. (B) Down-regulated genes. There was an overall effect of SD across both age groups in each gene. For Usp2 and Rbm3, aged animals had significantly higher levels of gene expression, although there was no interaction between SD and aging. For a given gene, (#) a significant overall effect of SD relative to NSD, (+) a significant overall effect of age, and (*) a significant post hoc comparison following a significant interaction between SD and age (P ≤ 0.05 was considered significant). Data are graphed as the mean ± SEM, and n = 8 for each group.