Fig. 5: fatty acid incorporation in Phytomonas serpens vacuoles. P. serpens was grown for two days in the presence of 5 μM labelled BODIPY-palmitic acid (green) and in the absence (A) or presence (B) of 50 μM tomatidine as described in Materials and Methods, then harvested and resuspended in phosphate buffered saline for fluorescence microscopy. Right panels are images obtained by interferential contrast microscopy. The inserts represents typical cells in detail. Bars = 10 μM; inserts = 0.25 μM.
